WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

PURIFICATION AND CHARACTERIZATION OF L-ASPARGINASE ENZYME FROM SPINACEA OLERACEA

Sarina P. Khabade*

ABSTRACT

L-asparaginase (E. C. 3.5.1.1) is an anticancer enzyme used as a drug to treat acute lymphoblastic leukaemia. In the present piece of work, Lasparaginase was purified from Spinacea oleraceae. The Lasparaginase enzyme was purified by salt precipitation (70%) followed by dialysis, anion exchange chromatography (DEAE- Cellulose) and gel filtration (sephadex G75) with final yield of 47.4% and purification fold 20.30. The molecular mass was found to be 160kda as estimated by SDS-PAGE. The enzyme activity of purified sample was found to be 322.3 IU and specific activity 1587.6╬╝moles/mg/min. This study aimed to determine novel source of L-asparaginase enzyme from Spinacea oleraceae, a possible solution for blood cancer.

Keywords: L-asparaginase enzyme, Spinacea oleraceae, acute lymphoblastic leukaemia.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More