PRODUCTION OF INULINASE BY THERMO-ALKALI TOLERANT STENOTROPHOMONAS MALTOPHILIA PSSB7 ISOLATED FROM AGRO-WASTES
Sulochana Mudgulkar Bhimsen*, Praveen Reddy Patil and Mohan Reddy K.
ABSTRACT
Thermo-alkali tolerant strain isolated from banana fields produced high
levels of inulinase with banana peel as carbon source and showed good
growth at pH 6.5 to 9.5 and 40oC to 65oC. It was identified as
Stenotrophomonas maltophilia PSSB7 by 16S rDNA analysis, and it is
deposited in NCL, Pune with strain number-NCIM 5323, with the
accession number- FJ707375. The optimal parameters for the
production of inulinase was as follows: pH 8.0, temp 45°C, higher levels
of inulinase (5551± 4.583 U/L) was achieved with 1% of banana peel.
And was found to be the best carbon source and the effect of different
substrates influenced the biosynthesis of inulinase indicating its role as
a constitutive enzyme in our strain. Sodium nitrate a best nitrogen
source and Mn+ was found to be the most suitable one. All these conditions make it a potential
strain for the industrial production from agro based wastes.
Keywords: Thermo-alkali tolerant strain isolated from banana fields produced high levels of inulinase with banana peel as carbon source and showed good growth at pH 6.5 to 9.5 and 40oC to 65oC. It was identified as Stenotrophomonas maltophilia PSSB7 by 16S rDNA ana
[Full Text Article]