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WJPR

World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.084

ICV : 90.8

WJPR Citation

	All	Since 2011
Citation	2903	2393
h-index	27	24
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Dr. Muhammad Baqir MR Fakhrildin
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Abstract

PRODUCTION OF INULINASE BY THERMO-ALKALI TOLERANT STENOTROPHOMONAS MALTOPHILIA PSSB7 ISOLATED FROM AGRO-WASTES

Sulochana Mudgulkar Bhimsen*, Praveen Reddy Patil and Mohan Reddy K.

ABSTRACT

Thermo-alkali tolerant strain isolated from banana fields produced high levels of inulinase with banana peel as carbon source and showed good growth at pH 6.5 to 9.5 and 40oC to 65oC. It was identified as Stenotrophomonas maltophilia PSSB7 by 16S rDNA analysis, and it is deposited in NCL, Pune with strain number-NCIM 5323, with the accession number- FJ707375. The optimal parameters for the production of inulinase was as follows: pH 8.0, temp 45°C, higher levels of inulinase (5551± 4.583 U/L) was achieved with 1% of banana peel. And was found to be the best carbon source and the effect of different substrates influenced the biosynthesis of inulinase indicating its role as a constitutive enzyme in our strain. Sodium nitrate a best nitrogen source and Mn+ was found to be the most suitable one. All these conditions make it a potential strain for the industrial production from agro based wastes.

Keywords: Thermo-alkali tolerant strain isolated from banana fields produced high levels of inulinase with banana peel as carbon source and showed good growth at pH 6.5 to 9.5 and 40oC to 65oC. It was identified as Stenotrophomonas maltophilia PSSB7 by 16S rDNA ana

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