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Abstract

STUDY ON PHYTOCHEMICAL SCREENING, TEST FOR INORGANIC ELEMENTS, QUALITATIVE AND QUANTITATIVE CHARACTERIZATION OF PHYTOCONSTITUENTS, TOTAL PHENOL, TOTAL FLAVONOID CONTENT AND ANTI-OXIDANT POTENTIAL OF METHANOL EXTRACT OF SCINDAPSUS AUREUS LEAVES

Kiran Kumar S. J.* and Deenadayalan K.

ABSTRACT

The aim of the present study was to identify the antioxidant potential
of methanol extract of Scindapsus aureus leaves. The extract was
examined for DPPH free radical scavenging activity, reducing power
capacity and for phenol and flavonoid content. Scindapsus aureus is
plant belonging to family Araceae. To give a scientific basis for usage
of this plant, the leaf extract were appreciated for its antioxidant
activity. In the present study we estimate the qualitative analysis and
the phytochemical compounds such as steroids, carbohydrates,
triterpenoids, coumarins, phenolic compounds, flavonoids, saponins,
nthroquinones, Proteins and lipids were screened in leaves of
Scindapsus aureus. Quantitative analysis of phenols, flavonoids and
Triterpenoids was further performed. Preliminary phytochemical screening of leaves were by
methanolic extracts which identified 11 major functional groups, amongst them flavonoid,
phytosterols, saponins and coumarins. Additionally inorganic elements like iron, chloride and
sulphate were identified by total ash analysis. The extract showed a dose dependent radical
scavenging effect in DPPH assay. IC50 for free radicals achieved by the extract is 144.72
μg/ml. The extract showed significant reducing power activity as compared to ascorbic acid
and proportionally increased with the increasing concentration of the extract. Increase in absorbance of the reaction mixture indicates the increase in the reducing power of the extract. Total phenolic and total flavonoid content has been present in the extract and the amounts are 16.42 ± 0.48 mg Gallic acid /g dry weight and 20.12 ± 0.13 mg Quercetin/g dry weight on dried extract respectively.

Keywords: Scindapsus aureus, phenol, flavonoid, antioxidant, DPPH, lignins, stilbenes, tannins, amines, betalains, flavonoids, quinones, coumarins, alkaloids.


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