ANTIOXIDANT STUDY OF ISOLATED CHEMICAL CONSTITUENTS FROM METHANOL EXTRACT OF THE CLERODENDRUM PHLOMIDIS LEAF
N. Habeela Jainab* and M. K. Mohan Maruga Raja
ABSTRACT
Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases. The medicinal value of plants lies in some chemical substances that produce a definite physiological action on the human body. The main aim of the study was to isolate active antioxidant chemical constituent/s from methanol extract of the Clerodendrum phlomidis leaf. Methanol extract of the Clerodendrum phlomidis was subjected to isolation by column chromatography. Nine compounds were isolated from the methanol extract of the Clerodendrum phlomidis leaf and were characterized by IR (KBr), 1H-Nuclear Magnetic Resonance (NMR), 13C-Nuclear Magnetic Resonance and Mass spectrometry. Isolated compounds were subjected to antioxidant activity by DPPH free radical scavenging assay. Compounds 6 (3,6,7-trihydroxy-2-(3-methoxyphenyl)-4Hchromen- 4-one) and compound 9 (Isopropyl linoleate) showed good antioxidant activity with an IC50 value of 63.16 μg/mL and 61.13 μg/mL respectively. The compounds 3-hexen- 1-yl benzoate, 2,3-dihydroxypropanal, 1-(2,4,5-trihydroxyphenyl)-1-butanone, 3-cyclohexen- 1-ol, isopropyl linoleate, oleic acid eicosyl ester are reported for the first time from Clerodendrum phlomidis leaves.
Keywords: Extraction, column chromatography, TLC, DPPH.
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