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WJPR

World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.084

ICV : 90.8

WJPR Citation

	All	Since 2011
Citation	2903	2393
h-index	27	24
i10-index	68	60

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World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
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Abstract

EFFECT OF SILVER NITRATE ON CALLUS CULTURES OF SPILANTHES ACMELLA MURR - AN ENDANGERED TOOTH ACHE MEDICINAL HERB

Sujana Papani* and Saivenkatesh Korlam

ABSTRACT

Efficient protocols for callus production from Spilanthes acmella were evaluated in the presence of ethylene antagonist AgNO3 in a callus inducing M S media supplemented with various concentrations of plant growth hormones ( IAA, 2,4-D, NAA and BAP). Addition of 0.2, 0.4 & 0.6 mg/L AgNO3 to the M S media significantly increased regeneration frequency and also produced more organogenic callus when compare to non-supplemented media. Among various concentrations administrated 0.2 and 0.4 mg/L AgNO3 were found to be most promising in producing 100% callus. Callus inducing media supplemented with 1.0mg/L NAA, 0.1 mg/L BAP And 0.4 mg/L AgNO3 found to be best suitable to produce green organogenic callus from hypocotyl explants of Spilanthes acmella. Administering M S media with high concentration of Auxins (2, 3 mg/L) promoted root formation rather than callus initiation.

Keywords: Callus, Ethylene inhibitor, AgNO3, Asteraceae, Spilanthol, Auxins.

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