EFFECT OF SILVER NITRATE ON CALLUS CULTURES OF SPILANTHES ACMELLA MURR - AN ENDANGERED TOOTH ACHE MEDICINAL HERB
Sujana Papani* and Saivenkatesh Korlam
ABSTRACT
Efficient protocols for callus production from Spilanthes acmella were
evaluated in the presence of ethylene antagonist AgNO3 in a callus
inducing M S media supplemented with various concentrations of plant
growth hormones ( IAA, 2,4-D, NAA and BAP). Addition of 0.2, 0.4
& 0.6 mg/L AgNO3 to the M S media significantly increased
regeneration frequency and also produced more organogenic callus
when compare to non-supplemented media. Among various
concentrations administrated 0.2 and 0.4 mg/L AgNO3 were found to
be most promising in producing 100% callus. Callus inducing media
supplemented with 1.0mg/L NAA, 0.1 mg/L BAP And 0.4 mg/L AgNO3 found to be best
suitable to produce green organogenic callus from hypocotyl explants of Spilanthes acmella.
Administering M S media with high concentration of Auxins (2, 3 mg/L) promoted root
formation rather than callus initiation.
Keywords: Callus, Ethylene inhibitor, AgNO3, Asteraceae, Spilanthol, Auxins.
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