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*Dr. Jikare Afreen, M. Parmar Hetal, Kokate Amruta, Pophle Prachi and Dr. Karad, Dilip


Direct electron transfer from electrodes to cells has many rewards over indirect electrical stimulation of microbial metabolism through electron shuttles or hydrogen production. In these study MFCs screened from soil samples near college campus (Shri Shivaji Mahavidyalaya, Barshi). From which four isolates (B1, B2, B3 and B4) were select for electricity production. These isolates were identified by morphological and biochemical studies. The identified isolates were Pseudomonas fluorescens, Bacillus pasteurii, Klebsiella ozaenae and Shigella spp. These MFCs were grown in two different medium i.e. Succinate medium and nutrient medium while it showed more electricity generation in succinate medium. The physicochemical parameters were optimized for more current formation. It was obtain that shaking condition was good for current production. The optimized condition showed that 5g/L succinic acid, 1g/L K2HPO4, pH 7 and 37oC are good conditions for current generation. After 72 hrs incubation period MFCs produced more electricity i.e. 1.5V and LED bulb had produced light.

Keywords: Succinate media, Nutrient medium and MFC.

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