PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIDIABETIC ACTIVITY OF MORINGA PTERIGOSPERMA LEAVES IN ALLOXAN-INDUCED DIABETIC RATS
Nishamol K. S.*, Dr. N. A. Aleykutty and Dr. Bindu A. R.
ABSTRACT
The objective of this research was to isolate the flavonoids from the
fresh leaves of Moringa pterygosperma Gaertn and evaluated for invivo
antidiabetic activity of ethanolic extract and the ethyl acetate
fraction on alloxan induced diabetic rat model.The fresh leaves of
Moringa pterygosperma Gaertn were subjected to extraction with
ethanol in a Soxhlet extractor for 10 hours. The ethanolic extract was
fractionated using solvents of increasing polarity like Petroleum ether,
benzene, chloroform, ethyl acetate and water. The preliminary
phytochemical analysis of each extract revealed the presence of
alkaloids, flavonoids, steroids, tannins, saponins and glycosides as
major components. The ethyl acetate fraction contains a high
percentage of phenolics and flavonoids. The flavonoid isolated by
preparative TLC was identified as rutin by HPTLC analysis. The ethanolic extract and ethyl
acetate fraction were screened for hypoglycemic efficacy in alloxan induced diabetic rats.
Both ethanolic extract and ethyl acetate fraction showed significant hypoglycemic activity as
compared to standard drug, metformin. The result obtained in this research work indicated
the promising effect of the hypoglycemic potential of ethanolic extract and ethyl acetate
fraction as compared to standard metformin. These results clearly showed that flavonoids
present in Moringa pterygosperma possesses potent hypoglycemic activity.
Keywords: Moringa pterygosperma Gaertn, Soxhlet extractor, phenolics, flavonoid, rutin, TLC, HPTLC, hypoglycemic, Metformin.
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