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Trupti Bajpai* and Maneesha Pandey


Background: Blood Stream Infections (BSI’s) are becoming the cause of increased hospital stay, health-care costs and high mortality rate among hospitalized patients. Aim: The present study was conducted with the aim of understanding the epidemiology of BSI and to raise the questions regarding the identification and reporting of pathogens and commensals/contaminants in culture positive blood samples. Materials & Methods: The present prospective study was conducted in the department of Microbiology of a teaching tertiary care hospital. A total of 1865 blood samples were processed through automated culture method. The samples that flagged culture positive were further processed through automated identification method. Results & Discussion: A total of 499 (26.7%) samples were found to be culture positive among which 51.8% isolates were Gram Positive bacteria, 42.3% were Gram Negative Bacteria and 5.7% were fungal (yeast) isolates. The isolates included several skin commensals and few rare/unusual emerging blood borne pathogens apart from the usual pathogens. After considering each isolate as a pathogen, it was identified up to the species level and reported to clinicians along with the note highlighting the basic and clinical features of the particular isolate. Useful lessons learned after analyzing the etiology of BSI helps the clinicians to understand the severe illnesses and the causative agents constantly associated with the disease. Conclusions: It is essential for the microbiologist to make the clinicians aware about the originality and severity of an organism so that he/she can corelate the reports clinically and proceed for the empirical therapy in the absence of sensitivity reports.

Keywords: Blood stream infection, automated blood culture, Vitek-2 Compact.

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