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Abstract

A STIMULATORY APPROACH FOR ACTIVATION OF FROZEN AND THAWED SPERM MOTILITY BY USING THEOPHYLLINE IN ASTHENOZOOSPERMIC MEN

Huda S. Abbas, Hayder A. L. Mossa* and Lubna A. Al-Anbari

ABSTRACT

Background: From each six couples, one is affected by infertility and half of these cases traced back to male. Sperm cryopreservation is a useful domain of Assisted Reproductive Technologies (ART's) used to preserve men fertility but unfortunately may be consequence of cryoinjury. Theophylline is an activating substance using to enhance and prolong fresh and thawed sperm motion by increasing a source of energy. Objective: This study was designed to evaluate whether in vitro sperm activation by Theophylline solution before cryopreservation improves percentages of sperm parameters post freezing-thawing process as compared with activation if done by the same substance but after cryopreservation. Subjects, Materials and Methods: Sixty male were participated in this study, twenty normozoospermic male and forty asthenozoospermic male. Semen samples were collected and seminal fluid analysis was done according to (WHO 1999, 2010). Each sample was divided into two aliquots, one of which was equally divided into two groups, the centrifugation was done to remove seminal plasma and then Swim-up technique was dependent for in vitro sperm activation by using FertiCult medium and Theophylline solution respectively prior to cryopreservation and one of which was also divided and activated by the same materials but post cryopreservation. Sperm parameters were measured in each activated group according to (WHO 1999). Results: using of FertiCult Flushing medium and Theophylline solution to activate fresh semen resulted in significant reduction in sperm concentration and also resulted in highly significant improvement (P<0.001) in percentage of sperm parameters in comparison with non-activated ones. However, Theophylline solution resulted in least reduction in sperm concentration and better results of progressive and non- progressive sperm motility also the percent of morphologically normal sperm (MNS). Pre-freeze activation of healthy control subjects and asthenozoospermic male patients did not improve post-thaw sperm parameters as compared when used these activation media post-freeze to activate post thawed sperm (non-activated), the later activation gave it significant increase (P<0.05) in percent of sperm motility and MNS. Conclusion: From the comparisons among both groups before cryopreservation and after cryopreservation, whatever treatment of semen is, the concentration of sperm will be reduced and sperm motility and MNS will be improved after activation with FertiCult Flushing medium and Theophylline solution. In both procedures, fresh semen and thawed semen, using of Theophylline resulted in the best increases in sperm motility and MNS percent.

Keywords: Asthenozoospermic, In vitro activation, Theophylline, Cryopreservation.


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