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Abstract

ISOLATION AND PURIFICATION OF A LECTIN FROM SEEDS OF A DROUGHT RESISTANT PLANT, CARAGANA GERARDIANA AND EVALUATION OF ITS ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES

*Aijaz Ahmad Ganaie, Ravi Prakash Mishra and Aashiq Hussain Allaie

ABSTRACT

A galactose-specific lectin was purified from seeds of a drought
resistant leguminous plant, Caragana gerardiana, by affinity
chromatography on lactose–agarose. Protein extract agglutinated
human erythrocytes (treated with proteolytic enzyme). Among various
carbohydrates tested, the lectin induced agglutination was inhibited by
D-galactose. SDS-PAGE showed that the lectin, named CGL produce
a single band establishing that the lectin is composed of similar type of
subunits. The lectin was reasonably thermostable showing full activity
within a temperature range of 20°C to 90°C. pH stability of the lectin
falls in the range of 2-9. The antimicrobial activity of the purified
lectin was carried out by agar ditch diffusion method at different
concentrations using appropriate standards. CGL demonstrated a
remarkable antibacterial activity against the pathogenic bacteria Pseudomonas aeroginosa,
Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. Antioxidant activity of the
purified lectins were carried out by DPPH- free radical scavenging activity (DPPH assay) and
Ferric (Fe3+) reducing power assay.

Keywords: Lectin, DPPH, Caragana gerardiana.


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