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Abstract

UV-VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF ASSAY OF ATENOLOL TABLET FORMULATION

Shinde G. S.*, R. K. Godge and Dr. N. S. Dighe

ABSTRACT

A simple, sensitive, specific and validated UV method has been developed for the quantitative determination of Atenolol in pure and tablet dosage form. The λmax was found to be 226 nm for assay. The linearity was found in concentration range of 0-150μg/ml. The correlation coefficient was found 0.999. The regression equation was found as y = 0.004x+0.007. The method was validated for linearity, accuracy, precision and System suitability. The LOD and LOQ for estimation of Atenolol were found as 2.088 &6.329 respectively. Recovery of Atenolol was found to be 99.12%.

Keywords: Atenolol, UV Spectrophotometry, Validation, Beer’s law.


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