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Abstract

HPLC STUDY OF GENUINE SAMPLE OF KATUKI (PICRORHIZA KURROA ROYLE EX BENTH) AND IT’S MARKET SAMPLE

Nagar Lalit*, Lamo Ringzin and Rath Sudipt Kumar

ABSTRACT

Introduction The importance of this drug Katuki has create a demand in the market but due to excessive deforestation and extreme weather conditions Himalaya is not able to meet the demand there by leading to unethical and dangerous practice of adulteration which seems to be the prime obstacle in excellence of the Ayurveda in this present Era. So it is mandatory to study the market samples to check the adulteration. Although chromatographic evaluation is overpriced criteria for the identification of drugs and developing countries like India may not afford these costly identification techniques. But, it may be essential sometimes in the competitive world. Hence chromatographic study is proposed to satisfy the needs of the country as well as the scientific society. Materials and methods Markets samples from six major markets of India and genuine sample of Katuki i.e. rhizomes of Picrorhiza kurroa from hills of Bhaderwa, dist. Kathua, State- Jammu and Kashmir were collected. Genuine sample of Katuki, lateral roots of Katuki and the most inferior quality sample from all the six markets were chosen for HPLC study. HPLC study was done in Amol Pharmaceuticals Private Limited Sitapura, Jaipur. Picroside II % in all the samples was calculated with the help of standard Picroside II. Results Genuine sample was having Picroside II 2.90% whereas Kolkatta sample and roots of Katuki had 1.47% and 0.54% respectively. Discussion HPLC results clearly shows that the genuine sample had almost double the amount of Picroside II as compared to the sample from kolkatta and 5.5 times more in comparison with the roots (Table-2). It also clearly shows that the roots are the adulterant and it will surely reduce the therapeutic potential of Katuki.

Keywords: Market study of Katuki, Adulteration, Picroside II, HPLC Study.


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