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Abstract

EFFECT OF CURCUMIN (CURCUMA LONGA) AGAINST HUMAN PULMONARY CARCINOMA CANCER CELLS: AN IN-VITRO COLONY FORMING AND APOPTOSIS ASSAY

Dilla Jose* and P. Senthilkumaar

ABSTRACT

The present study focused the anticancer effect of Curcumin against
Pulmonary Carcinoma (NCI-H292) through colony forming and
apoptosis assay. In addition, Paraquat act as an inducer. Different
concentrations (1, 5, 10, 25, 50 and 100 μg/ml) of Curcumin and
Paraquat were used. In colony forming study, colonies were stained
with crystal violet and the number of clones in a given area was
counted for each condition. But in apoptosis assay, the study was
determined by measuring Annexin V activity using Annexin V
apoptosis kit. This study witnessed that higher concentration of the
curcumin gave a good results against cancer cell lines.

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