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Chandrakala Parajuli* and Prof. Dr. Dharma Prasad Khanal


The main objective of current study was to find out the presence of anti-inflammatory activity and perform brine shrimp bioassay in the methanol extract of Aleuritopteris anceps (Ranisinka). The plant was collected from Kathmandu and Lalitpur district about 1300 meter altitude in months June to July. The plant was subjected to hexane, ethyl acetate and methanol extraction by maceration process. Previous studies on qualitative analysis revealed that different extract of the fern offered diverse phytochemical compounds such as alkaloids, saponins, glycosides, terpenoids, tannins, carbohydrates and phenols. This plant also possesses different biological activity such as antioxidant, antimicrobial sensitivity test and anti-diabetic activity. The methanol extract of fern showed mild cytotoxic activity with LC50 50.118 μg/ml. There was dose dependent anti-inflammatory activity shows by 200mg/kg and 400 mg/kg concentration of methanol extract comparing with standard drug Indomethacin after 5 hour of egg albumin induce paw edema. The percentage of anti-inflammatory activity of 200mg/kg was found 24.31% 400mg/kg extract showed 26.18% where as standard Indomethacin showed 31.79%. Thus Aleuritopteris anceps showed significant anti-inflammatory activity.

Keywords: Aleuritopteris anceps, Ranisinka, Brine shrimp bioassay, Cytotoxic activity, Anti-inflammatory activity.

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