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Abstract

EFFECT OF CARBON, NITROGEN, PROTEIN SOURCES AND INCUBATION TIMES AND TEMPERATURE ON POLYHYDROXY BUTYRATE SYNTHESIS BY BACILLUS SUBTILIS MTCC 1790.

Dr. J. Jeyasree*, D. Praba, Dr. S. Jawahar and G. Kalidhasan

ABSTRACT

Polyhydroxybutrate(PHB), polyhydroxyalkanoate a polymer belonging to the polyesters class that was first isolated and characterized in 1925 by Indian microbiologist Abhilash singh. To study of this paper identified the PHB granules in may be either renewable(based on agricultural, plants or animal products) or synthetic. There are four types of biopolymer based respectively on starch, sugar, cellulose and synthetic materials. The maximum growth of Bacillus subtilis KO strain was from 0.608 to 0.780g/10ml broth medium recorded at molasses concentration of 10%w/v at 45˚calcias and 24hrs.Inoculation period when molasses broth medium supplement with 0.1%w/v of ammonium sulphate as a nitrogen source. Our findings confirm the fact that Bacillus subtilisKO strain succed to growth strongly in the absence of any of the inorganic nitrogen source supplied to the molasses broth medium. Bacillus subtils MTCC1790 culture was used for the production of PHB a different media composition. A number of Bacillus subtilis have been reported to accumulate 9 to 67% cell dry weight(CDW) of PHB. Anaerobic system also have the added benefit of potentially co-producing other valuable by products like ethanol, lactate, succinate or hydrogen.

Keywords: Polyhydroxybutarate(PHB), Polyhydroxyalkanoate(PHA), Bacillus subtilis.


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