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Mital K. Aadesariya, Bhagwati M. Gauni, Srinivas M. Duggirala, Vijay R. Ram*, Suhas J. Vyas


Plants have been proven to be a rich source of diverse class of
antimicrobial molecules useful for treatment of various infections.
These purified or crude antimicrobial constituents are obtained from
plants by extraction using a range of organic solvents. Their
antimicrobial or inhibitory effects on various microbes can be checked
by agar well diffusion method. In this study we have focused on
assessment of antibacterial activity of leaf extracts of Abutilon
pannosum (APL) and Grewia tenax (GTL) using four different
solvents i.e., Milli-Q, methanol, petroleum ether and isopropanol by
the agar well diffusion method. The plant extracts were prepared by
successive extraction method and tested against eleven different human
pathogenic bacteria. The test organisms included Gram-positive organisms such as
Staphylococcus aureus, Enterococcus fecalis, Streptococcus epidermidis, Streptococcus
pneumoniae and Gram-negative organisms; Escherichia coli, Salmonella typhi, Serratia
marcescens, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus vulgaris. Various
solvent extracts of both plant materials prepared at an initial concentration of 1 mg/mL
showed variable degree of inhibitory activity against tested pathogens. The results showed
that both the plant extracts have very good inhibition against S. pneumoniae and S. typhi,
moderate inhibition against E. fecalis, S. epidermidis, and P. aeruginosa while they could not inhibit the growth of E. coli, S. aureus, S. marcescens, K. pneumoniae, B. subtilis and P. vulgaris. The results were also compared with standard antibiotics for Gram-positive and Gram-negative organisms.

Keywords: Antibacterial activity, Grewia tenax, Abutilon pannosum, Agar well diffusion.

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