WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
            Best Paper Award :
Dr. Muhammad Baqir MR Fakhrildin
Download Article: Click Here

Search

Track Your Article

Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF METFORMIN HYDROCHLORIDE BY RP-HPLC IN DRUG SUBSTANCE

Merugu Manasa* and Peddiboina Gopi

ABSTRACT

A simple, accurate, precise and rapid Reversed-Phase High Performance Liquid Chromatographic (RP-HPLC) method has been developed and subsequently validated for the estimation of Metformin Hydrochloride in pure form. The proposed method was separation of Metformin by reversed-phase mode using SHISEIDO C-18 analytical column (250×4.6 mm I.D., 5 μm particle size) and mobile phase consisted of Acetonitrile: Phosphate buffer PH-7(50:50 v/v). Flow rate of 1.0 ml/min and UV detection was set at 254 nm were selected peak produced for Metformin Hydrochloride at retention time of 2.27 min. The method was validated according to ICH guidelines. Linearity was obtained in the concentration range of 10-35 μg/ml. Mean percent recovery of samples at each level for drug was found in the range of 100.93%, %RSD was 1.47, LOD and LOQ were 0.011179 & 0.033876 respectively. By change in parameters like flow rate and temperature system suitability parameters were within the limit, it indicates that the method was robust.

Keywords: Metformin Hydrochloride, HPLC, Method development, Validation, Acetonitrile, Phosphate buffer and C-18 column.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More