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Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF NIFEDIPINE IN FORMULATION BY RP-HPLC

Tejaswini*, Ch. M. M. Pradada Rao and D. Dhachinamoorthi

ABSTRACT

The developed method was a simple, efficient, economical method for the Validation of Nifedipine reverse phase high pressure liquid chromatography and UV spectrophotometer. For Nifedipine Impurities Chromatography was performed on Intersil C18-column (4.6×250mm, 5μm) with mobile phase TBH: Acetonitrile 25:75 (v/v) at a flow rate of 1.0 mL/min and eluents were monitored at 238 nm The percent recoveries ranged between 92.1 – 108.2 %,. RSD for all Parameters are within the acceptance. The method could be successfully used for the analysis Nifidepine in both API and Marketed tablet formulations.

Keywords: Nifidipine, TBH, Acetonitrile, Intersil, API.


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