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Abstract

ANTIMICROBIALS SUSCEPTIBILITY PATTERN OF BACTERIA ISOLATED FROM SEMINAL FLUIDS OF INFERTILE SUDANESE MEN AT ANDROLOGY CLINIC, KHARTOUM STATE, SUDAN

Sharaf Eldeen R. I.*, Kafi S. K., Badawi S. B., Musa H. A. and Eljack M. S.

ABSTRACT

Background: Bacterial infections play a role in the etiology of male infertility since bacteria can agglutinate and hence immobilize spermatozoa. This study aimed to determine bacterial species that cause semen infection and their antimicrobial susceptibility among infertile Sudanese men in Khartoum State. Methods: A total of 518 infertile men were recruited to participate in this study. Semen specimen was collected from each participant either by masturbation or coitus interruptus in a sterile container. The semen specimens were incubated as follows: Blood agar and MacConkey agar plates aerobically and the Chocolate agar plates under 5%-10 Co2 concentration using candle jar at 37°C for18-48 hours. Plates with significant bacterial growth were identified by the conventional bacteriological techniques and then antimicrobial susceptibility test was done for each identified organism using Clinical Laboratory Standards Institute (CLSI) techniques. The selected antibiotics used for the sensitivity test were Gentamicin, Co-trimoxazole, Ciprofloxacin, Ceftriaxone, Pencicillin, Fusidic-acid, Chloromphenical, Nitrofurantoin Augmentin (Co-amoxiclav), Erythromycin, Vancomycin, Cftazidime and Cefuroxime. Results: Of the total number of specimens, 431 (83.8%) revealed significant bacterial growth. A considerable percentage (48.0%) of the isolates were gram positive. The most common bacterial species isolated were Staphylococcus saprophyticus (42.2%), Neisseria gonorrheae (33.5%) and Staphylococcus aureus (12.1 %). The susceptibility of all gram positive and gram negative isolates to Gentamicin was (62.6% and 58.7%) respectively. 59.5% of Neisseria gonorrheae isolates were sensitive to Gentamicin and 49.5% to Ceftriaxone. Staphylococcus saprophyticus isolates showed 69.1% sensitivity to Gentamicin, 50.8% to Chloromphenical and 50.0% to Ciprofloxacin. Conclusion: Gentamicin was found to be the best antibiotic for both gram positive and gram negative bacteria and can be used empirically to treat men with semen bacterial infection.

Keywords: Bacterial isolates, Semen, antibiotic susceptibility, infertility, Sudan.


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