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Mallapu E. Rani*


This paper describes the validation of a new spectrophotometric method for the assay of oxyfedrine as in pharmaceutical dosage forms, the DDQ method employs for the estimation of drugs containing amino group and phenolic groups. In this method, the reaction of oxyfedrine with 2,3 – dichloro -5-6 – dieyano -1,4 benzoquinone (DDQ) inchloroform mixture forms honey colour – charge – transfer complex. The honey colour charge – transfer complex solution was stable for more than 24 hrs. The absorbance of the honey colour solution was measured at the wave length 470mm against the regent blank. The calibration graph was obtained by plotting absorbance values against the concentrations of oxyfedrine solution. The calibration curve was found to be linear over a concentration range of 50 to 250 g / ml of oxyfedrine. The linearity of the curve obtained indicate that it obeys Beer’s law. Results of analysis were validated statistically and by recovery studies. The procedures described were successfully applied to the determination of oxyfedrine in tablets.

Keywords: D.D.Q method by speetrophotuetrically oxyfedine, Method of validation.

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