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Abstract

FORMULATION, EVALUATION AND QUANTIFICATION OF DEACEYL GYMNEMIC ACID AND GALLIC ACID BY USING HPLC AND HPTLC OF ANTIDIABETIC POLYHERBAL GRANULES

Raseda G. Kureshi* and Mariyan R. Patel

ABSTRACT

The aim of present research work is to formulate and standardize Gymnema sylvestre containing polyherbal formulations. Gymnema sylvestre widely used as an anti-diabetic drug Gymnema sylvestre and other herbal drugs that having anti diabetic activity like Momordica charantia, Emblica officinalis, Enicostemma littorale, Trigonella foenum-graecum, Azadirachta indica, Picrorhiza kurroa were formulated into polyherbal granules by wet granulation technique and then evaluated for various parameters like angle of repose, bulk density, tapped density, car’s index and hausner’s ratio. The formulated granules exhibited excellent flow properties compared to marketed formulation. Quantification of marker compounds present in various formulations done by HPTLC and HPLC. DAG-1 (Deacyl gymnemic acid) and Gallic acid content were found to be the highest in lab formulation and lowest in market formulation.

Keywords: Poly herbal, anti -diabetic activity, Gymnema sylvestre, wet granulation, DAG, Gallic acid.


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