Abstract

COMPARATIVE GC-MS ANALYSIS OF LEAF AND ROOT EXTRACT OF MEDICINAL PLANT WITHANIA SOMNIFERA

Indra Rautela*, Manish Dev Sharma, Nishesh Sharma, Kunal Kishor, Keerti Singh, Narotam Sharma

ABSTRACT

Medicinal plants are enormous significance in the present research, drug industries depend directly and indirectly in medicinal plants for the production of pharmaceutical compounds. Withania somnifera is important medicinal plant having sources for many chemicals used as pharmaceutical chemicals. Present study is based on identification of bioactive compounds from leaf and root of Withania somnifera in ethanol and methanol solvent system by using Gas chromatography and Mass spectroscopy (GC-MS). GC-MS analysis of methanolic and ethanolic leaf extract was done and found 35 and 49 phytochemical compounds respectively. For the root both solvent system was used and found 68 and 29 phytochemical compounds. The GC-MS analysis revealed the presence of various compounds such as withanolide B, rosifoliol, dodeconoic acids, hexadecanoic acid Guanosine, squalene, phytol, retinol and sitosterol etc in the leaf and root of W. somnifera. Hence, the Withania somnifera may have anticancer, anti-microbial activity, antioxidant and anti-diabetic activity due to the presence of secondary metabolites.

Keywords: Withania somnifera, phytochemical, anti-diabetic.