COMPARATIVE GC-MS ANALYSIS OF LEAF AND ROOT EXTRACT OF MEDICINAL PLANT WITHANIA SOMNIFERA
Indra Rautela*, Manish Dev Sharma, Nishesh Sharma, Kunal Kishor, Keerti Singh, Narotam Sharma
ABSTRACT
Medicinal plants are enormous significance in the present research,
drug industries depend directly and indirectly in medicinal plants for
the production of pharmaceutical compounds. Withania somnifera is
important medicinal plant having sources for many chemicals used as
pharmaceutical chemicals. Present study is based on identification of
bioactive compounds from leaf and root of Withania somnifera in
ethanol and methanol solvent system by using Gas chromatography
and Mass spectroscopy (GC-MS). GC-MS analysis of methanolic and
ethanolic leaf extract was done and found 35 and 49 phytochemical
compounds respectively. For the root both solvent system was used
and found 68 and 29 phytochemical compounds. The GC-MS
analysis revealed the presence of various compounds such as withanolide B, rosifoliol,
dodeconoic acids, hexadecanoic acid Guanosine, squalene, phytol, retinol and sitosterol etc
in the leaf and root of W. somnifera. Hence, the Withania somnifera may have anticancer,
anti-microbial activity, antioxidant and anti-diabetic activity due to the presence of
secondary metabolites.
Keywords: Withania somnifera, phytochemical, anti-diabetic.
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