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Abstract

IN VITRO MICROPROPAGATION FROM NODAL EXPLANT OF KNOCK OUT ROSE- AND EVALUTION OF ITS ANTIBACTERIAL ACTIVITY

*B. Janarthanam and T. Sekar

ABSTRACT

A protocol has been developed for in vitro plant regeneration from a nodal explant of Knock out rose variety, an economically important variety for cut rose. The present study utilized 6-benzyl adenine (BA) and napthaleneacetic acid (NAA) for the induction of shoot organogenesis on nodal explants. The highest frequency of shoot regeneration (71.67± 2.9 %) and number of shoots per explant (8.00 ± 1.00) were obtained on medium supplemented with 4.44 μM BA and 0.14 μM NAA. Rooting was achieved on half strength MS solid medium supplemented with 2.46 μM indole-3-butyric acid (IBA) produced (6.0 ± 1.0) roots with an average height of 4.9 ± 0.26 cm after 30 days of culture. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farmyard manure (1:1:1). Antibacterial activity of ethanolic flower extract was measured by agar disc diffusion method. Different concentrations of ethanolic flower extract of Knock out rose was tested for the anti-bacterial activity. Ethanolic flower extract of Knock out rose showed the maximum zone of inhibition (17 mm) and (15 mm) against Pseudomonas aeruginosa and Escherichia coli respectively.

Keywords: Knock out rose, Micropropagation, Nodal explant, Antibacterial activy.


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