IN VITRO CYTOTOXIC ACTIVITY AND PRELIMINARY PHYTOCHEMICAL ANALYSIS OF THE CRUDE EXTRACTS OF ELEUTHERINE BULBOSA (MILLER), URBAN
Rani V. S.*
ABSTRACT
Eleutherine bulbosa (Miller) Urban is an exotic plant with known
medicinal uses. It is a tropical American plant, now naturalized in
Asia. In Kerala and Southern parts of Tamil Nadu, the underground
bulb of the plant is known by the local names, ‘Vizhanarayani’ or
‘Neerotikizhangu.’ The present study focuses on the in vitro cytotoxic
activity of crude bulb extracts against Dalton’s Lymphoma Ascitic
(DLA) tumour cell lines, using Tryphan blue exclusion method.
Among the different crude extracts, only the crude hexane and ethyl
acetate extracts showed potent cytotoxic activities towards DLA
tumour cell lines. The chloroform and methanol extracts did not show
any cytotoxicity to DLA tumour cell lines. The LC50 value of crude
hexane extract was 67.97μg/mL. The crude ethyl acetate extract showed LC50 value at a
concentration of 41.02μg/mL. At a higher concentration (100μg/mL), the ethyl acetate extract
showed a much more cytotoxic activity (80% cell death) compared to the hexane extract
(64% cell death).
Keywords: Eleutherine bulbosa, DLA tumour cell line, quinones, cytotoxicity, Tryphan blue.
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