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*Rachana Dubey and Nameeta Bende


Like other transition metal ions, Fe2+ forms many coordination compounds in which the iron ion is surrounded by various Lewis bases, called ligands. Many of these coordination compounds are colored, unlike aqueous ferrous ion itself. If the colored complex is stable and forms quantitatively, it can be used to measure of the amount of iron present. 1,10-Phenanthroline or O-Phenanthroline is a bidentate chelating agent, which forms a stable red complex with ferrous iron. This procedure is selective for Fe2+, even in the presence of other metals, for several reasons. Sodium acetate buffer was used to maintain the pH for optimum complex formation while Hydroxylamine Hydrochloride was added as a reducing agent to intercept oxygen and prevent oxidation of ferrous iron to ferric iron. Fe(II) + 3 phen ⇔ Fe(phen)3 2+ (Red) Determination of Iron in Natural and synthetic samples was carried out using the UV Visible Spectophotometer CL- 320 (Chemi line). Concentration of iron in test solution was calculated from the Standared Curve prepared. For each natural and synthetic samples at least two readings were obtained and the average calculated. Result of the study suggests theta method can be used satisfactory for the analysis of iron.

Keywords: UV-Visible Spectrophotometer, Natural sample, Iron test and Analysis.

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