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Abstract

ASSESSMENT OF AQUEOUS LEAF EXTRACT OF FICUS RACEMOSA L. FOR VASCULOGENESIS AND ANGIOGENESIS USING IN VIVO MODEL OF CHICK CAM ASSAY

Aruna A. Kanase*, Anuya S. Mane, Shilpa M. Pachapurkar and Jaywant J. Jadhav

ABSTRACT

The influence of aqueous extract of Ficus racemosa L. leaf was evaluated, using in vivo model of CAM assay with window method, to study the pro/antiangiogenic and vasculogenic properties. The doses were administered in HBSS at 48, 72 and 96 hrs in twelve groups (each of the incubation interval has 4 groups viz., normal, sham operated, HBSS control and experimental and each group contained 6 eggs in development). The observations include photographic data and quantitative analysis of primary, secondary and tertiary vessels, that were observed after 144 hrs for each of the incubation interval groups. The results indicate strong antiangiogenic effects of the aqueous extract directly proportional to the hours at which administration was carried out.

Keywords: Ficus racemosa L., leaf extract, Vasculogenesis, angiogenesis, CAM assay.


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