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Abstract

PRODUCTION OF ANTIMICROBIAL METABOLITES BY STREPTOMYCES PUNICEUS LC13

Tanupriya Ojha* and Kantishree De

ABSTRACT

Objective: Actinomycetes are a source of wide variety of secondary metabolite producers in drug discovery programs, accounting for approximately 30% of the total microorganisms in the soil rhizosphere. Streptomycetes puniceus LC13 strain was isolated from rhizosphere soil of Lantana camara, Jabalpur (M.P.), India and it was produce number of bioactive metabolite which was capable to discover novel drug formulation. Methods: The compounds were purified by solvent extraction, silica gel column chromatography (60-120 mesh) and Thin layer chromatography (TLC). The structures of the compounds were elucidated by Gas Chromatography and Mass Spectroscopy (GC-MS). Results: The results indicated that the Streptomyces puniceus LC13 produced seven peaks which have antimicrobial activity with Rf valve 0.49. Conclusion: It was concluded that rhizosphere soil is a promising source of secondary metabolites with highest antimicrobial activity.

Keywords: Streptomycetes puniceus, Lantana camara, GC-MS, antimicrobial activity.


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