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Abstract

DESTAINING POTENTIAL OF BACTERIAL LIPASE ENZYME ISOLATED FROM PROVIDENCIA RETTGERI INHABITING THE FRESH WATER FISH MYSTUS BLEEKERI

N. Deepa, R. Usha, A. Anbarasu, K. Anuanandhi, P. Karnan and K. Elumalai*

ABSTRACT

The objectives of present study were to isolate and identify lipase
producing bacterial strain Providencia rettgeri (MB3) was isolated
from fresh water fish Mystus bleekeri. Total of 6 bacterial strains were
obtained in the fish tissues. Screenings of lipase production were tested
on tributyrin agar plate. Among the 6 isolates, MB3 was showed
maximum zone of hydrolysis around the well. Partial purification of
crude enzyme produced from P. rettgeri (MB3) was carried out by
ammonium sulfate precipitation and dialysis further purified for
DEAE-Cellulose column chromatography. The optimization of lipase
enzyme was active at pH 8, temperature 40°C, incubation 48 hrs,
carbon source as lactose and best nitrogen source was peptone.
According to our knowledge, this study demonstrated the first report on alkaline lipase
producing to P. rettgeri (MB3) isolated from fresh water fish. The partially purified lipase
enzyme was effectively removed by the blood stains and paves the way for its possible
utilization in textile industry in the near future.

Keywords: Lipase enzyme, Providencia rettgeri, Mystus bleekeri, Optimization, Blood stain.


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