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Abstract

SCREENING OF SECONDARY METABOLITES, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF CEROPEGIA BULBOSA EXTRACTS'

*Somashekhar R.

ABSTRACT

Phytochemicals are the secondary metabolites produced from all the plants, but some of the secondary metabolites have high medicinal value. To evaluate the medicinal properties from Ceropegia bulbosa, phytochemicals extracted from different solvents such as ethyl acetate, acetone, ethanol, hot water and cold water. The qualitative estimation of phytochemical revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoids, quinines, cardiac glycosides and phenols. Antibacterial activity was analyzed by Resazurin method, results revealed that ethanol extracts showed strong antimicrobial activity. Antifungal activity was carried out using well diffusion method, maximum antifungal activity was observed also in ethanol extract. Antioxdant activity was analysed using DPPH, ABTS and Reducing power, results reveals that, ethanol extract showed for DPPH assay, but hot water showed both ABTS and Reducing power assay. Antidiabetic activity was found in hot water extracts based on IC 50 value of Amylase inhibition assay.

Keywords: Phytochemicals, Antibacterial activity, Resazurin, DPPH, ABTS.


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