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Saket A. Deshmukh*, Yogesh N. Gholse, Rahul H. Kasliwal and Dinesh R. Chaple


Aqueous leaf extract of a tropical variety of Psidium guajava L. (Myrtaceae), Azadirachta indica (Meliaceae) & Glycyrrhiza glabra (Leguminosae) was used to formulate fifteen batches of herbal mouthwash. The antimicrobial potentials of these herbal mouthwash formulations in oral hygiene were assessed in vitro using a modification of the conventional methods for evaluating oral antiseptics. Formulations containing Psidium guajava, Azadirachta indica & Glycyrrhiza glabra leaf extract and a standard mouthwash (Povidone iodine, Chlorhexidine & Geofresh) served as test samples and controls respectivelly. The mouthwash formulations were screened for antimicrobial activities against cultures of Staphylococcus aureus, Escherichia coli and Bacillus subtilis. The extinction time of each formulation batch was determined against each test organism. The fifteen batches containing aqueous extract showed a high level of activity against the test organisms- E. coli and S. aureus comparable to the activity shown by the standard mouthwash (Povidone iodine, Chlorhexidine & Geofresh). The study encourages further stability and Jn vivo assessment to develop P.guajava, A. indica & G. glabra leaf extract as an ingredient of commercial mouthwashes.

Keywords: Antimicrobial, Herbal Mouthwash, Psidium Guajava, Azadirachta, Indica, Glycyrrhiza Glabra.

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