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Abstract

INVITRO ANTI-OXIDANT AND ANTI-INFLAMMATORY PROPERTY OF ETHANOL EXTRACT OF TRIDAX PROCUMBENS LEAVES

Sedhuraman S.*, Balaji A., Amerthiya D., Kavya G., Meena C., Lironisha L., Kannikaparameswari N.

ABSTRACT

The present investigation was aimed to evaluate the in vitro antioxidant and anti-inflammatory property of leaves of Tridax procumbens.L by DPPH assay and membrane stabilization assay. Action was observed in dose dependent manner. The result shows that in DPPH assay 250 μg/ml of chosen extract, Tridax procumbens Ethanol Extract (TPEE) showed maximum protection 45% and standard drug provided 69% protection. Similarly in membrane stabilization assay the selected extract at concentration of 500 μg/ml showed maximum results 78% and standard drug Diclofenac sodium provided 58% protection. In additional, phytochemical analysis of Tridax procumbens showed the presence of alkaloid, flavonoid, tannin, saponin, amino acid and sugars. It reveals that the phytochemical constituents are responsible for anti-oxidant and anti-inflammatory property. The future work will be determination of anti-oxidant and anti-inflammatory activities by in vivo methods.

Keywords: Tridax procumbens; in vitro anti-oxidant; DPPH assay; in vitro antiinflammatory property; membrane stabilisation assay method.


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