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Abstract

ESTIMATION OF TOTAL PHENOLIC, FLAVONOID AND ANTIOXIDANT OF METHANOLIC EXTRACT OF THALASSIOSIRA WEISSFLOGII

Ashashalini, M. Syed Ali*, V. Anuradha, V. Suganya and P. Bhuvana

ABSTRACT

Marine algae contain a wide assortment of bioactive compounds, which have viable applications in pharmaceutical, medical, cosmetic, nutraceutical, nourishment and agricultural fields. Natural antioxidants, found in Diatoms show the vital role against numerous diseases and ageing progressions through assurance of cells from oxidative damage. The in vitro antioxidant assay, total phenolic content and total flavonoid content of methanolic extract of Thalassiosira weissflogii were performed along with standard drug like ascorbic acid, BHT, Gallic acid etc., Diatom Thalassiosira weissflogii was extracted using methanol solvent. The maximum phenolic content and flavonoid content of test sample was 11.34 and 2.06 mg equivalent to standard drug. All other antioxidant activity like DPPH, Nitric oxide, SOD, SO, LPO and β carotene shows better result than the standard drug. Thus, the result suggested that methanolic extract diatom Thalassiosira weissflogiicould lead their application in many useful health cares also in chemoprevention of a variety of diseases including cancer.

Keywords: Total phenolic content, total flavonoid content, diatom Thalassiosira weissflogii, DPPH and reactive oxygen species.


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