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R. P. Meena*, S. C. Verma, A. S. Khan, S. A. Ansari and S. Arifin


In Unani System of Medicine, though the single drugs are used for many ailments, but compound formulations plays a major role in the treatment of various disorders. Evaluation of pharmacopoeial studies for herbal formulations is an essential part to assess the quality of drugs for therapeutic value. The World Health Organization (WHO) has given a detail protocol for the evaluation of herbal single drugs, but very little literature is available for the standardization of poly-herbal drugs. Majoon-e-Azaraqi is an ancient most commonly used Unani formulation prescribed for the ailments of Nerve strengthening, Hemiplegia, Luqwa, Facial paralysis, Tremor, Trembling, Rheumatism, Epilepsy and Neurasthenia. The poly herbal drug Majoon-e-Azaraqi was prepared in different batches with the combination of 15 ingredients. Three different batch samples prepared at laboratory scale were subjected to evaluate the physico-chemical, TLC/HPTLC finger prints, heavy metal, microbial load, aflatoxins and pesticide residues. The physico-chemical data such as moisture content was 19.52%, alcohol soluble extractives 32.66% and water soluble extractive 70.65% shows presence of polar compounds and inorganic materials respectively. The content of total ash was 1.44% and acid insoluble ash 0.34% shows negligible amount of siliceous matter present in the drug. TLC/HPTLC finger prints of chloroform and alcohol extracts were developed with the developing systems toluene: ethyl acetate - 9:1 and 6:4 respectively. All three different batch samples were found to be safe when tested for the heavy metal content, microbial load, aflatoxins and pesticide residues. This research work may be adopted for laying down the pharmacopoeial standards and TLC/HPTLC finger prints for Majoon-e-Azaraqi formulations.

Keywords: Majoon-e-Azaraqi, Pharmacopoeial Standardization and HPTLC analysis.

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