Abstract

HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY FINGERPRINTING OF THE ALKALOIDS FROM AILANTHUS EXCELSA (ROXB.) LEAVES

Ranjana Sharma, Sudhir Singh Gangwar, Amita Tilak and Ravindra C. Sutar*

Abstract

Objective: The present study was conducted to identify the alkaloids from methanol extract of medicinally and economically useful leaves of Ailanthus excelsa (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of alkaloids of methanol extract of leaves revealed at Rf 0.31 orange-red band color, Hence alkaloids are detected. Conclusions: With the results of HPTLC analysis and above Rf value we have concluded the presence of alkaloids in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, alkaloids, HPTLC Fingerprinting.