Abstract

THE GROWTH SUPPRESSING ACTIVITY OF SPATHODEA CAMPANULATA BARK ON C6 & U87MG INVOLVE INDUCTION OF APOPTOSIS AND CELL CYCLE ARREST

Shrawan Kumar and Shila Elizabeth Besra*

Abstract

The anticancer activity of pant extract has already reported. We evaluated the cytotoxic and apoptosis effect of bark extract of Spathodea campanulata, on glioma cell lines. It was found that extract significantly decreased viable cell and caused apoptosis, which is further confirmed by morphologically. Gel electrophoresis study shows fragmented DNA in the form of multiple bands and Flow cytometric analysis revealed appreciable number of cells in early & late apoptotic stages. A significant change in the mitochondrial membrane potential was obtained in the BESC treated glioma cells with MMP shift assay. The cells were arrested mostly in G0/G1 phase of cell cycle.

Keywords: Bark, Spathodea campanulata, Glioma, Apoptosis, Cell Cycle.