Abstract

PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND IN VITRO STUDY OF ANTIOXIDANT ANTIMICROBIAL AND ANTICANCER ACTIVITIES OF ETHANOLIC EXTRACT OF IPOMOEA QUAMOCLIT

K.Renuka*, K.Ravishankar

Abstract

The aim of the present study is to investigate the phytochemical constituents, antioxidant antimicrobial and cytotoxic activity of ethanolic extract of Ipomoea quamoclit. Preliminary phytochemical tests revealed the presence of alkaloids, tannins, saponins, flavanoids, glycosides, phytosterols, carbohydrates, aminoacids, phenolic compounds. The antioxidant activity of Ipomoea quamoclit was evaluated by 1,1-Diphenyl-2-Picryl Hydrazyl free radical scavenging activity, phosphomolybdenum method ,reducing power method, scavenging of nitric oxide and the IC50 values for the Ipomoea quamoclit and reference standard Gallic aid were found to be(68.4μg/ml,0.82μg/ml for DPPH method),(96.4μg/ml,2.44μg/ml for nitric oxide method).Antimicrobial activity was evaluated by Agar Well Diffusion method. In vitro screening of Ipomoea quamoclit ethanolic extract showed species specific activity in inhibiting growth of bacteria and fungi. The ethanolic plant extract showed good activity against selected gram-positive bacteria (Bacillus subtilis,Staphylococcus aureus, Staphylococcus werneri)gram-negative bacteria (Pseudomonas putida,Escherichia coli, Pseudomonas aeruinosa,Proteus mirabiis,Klebsiella pneumonia)and fungal strain (candia albicans).The observations revealed significant zone of inhibition and supports to antimicrobial activity. The cytotoxic studies indicate the Ipomoea quamoclit inhibit caco2 (colon cancer) cell viability in a dose dependent manner. The IC50 values calculated were >100μg/ml for ethanolic plant extract, 22.12μg/ml for standard drug 5-fluorouracil.These findings revealed that the ethanolic extract of Ipomoea quamoclit has cytotoxic, antioxidant and antimicrobial activities. The bioactive compound responsible for these activities could be isolated and identified to develop a new drug.

Keywords: Antioxidant, anticancer, caco2 cell line, agar well diffusion.