Abstract

ANXIOLYTIC AND ANTICONVULSANT ACTIVITIES OF EXTRACT OF CARISSA CARANDAS LINN. LEAVES

Parikshit V. Choudhari*, Dr. Vanita G. Kanse, Dr. A. Venkatachalam and Punam R. Pal

Abstract

The aim of this analysis was to look into anti-anxiety properties of various Carissa carandas leaf extracts. Leaves of plant were extracted with solvents ranging from petroleum ether (60-80oC), chloroform, ethyl acetate, ethanol, in order of increasing polarity. Using elevated plus maze apparatus; both of rudimentary extracts were tested for antianxiety function in mice. Among these extracts, ethyl acetate ethanolic extract demonstrated substantial anti-anxiety effects in mice at 400 mg/kg as compared to regulation normal (diazepam, 4 mg/kg). Ethyl acetate Ethanolic extract increased time spent amount of arm entries in open arms of elevated plus-maze when given at dosage of 400 mg/kg. Since phytochemical screening of ethanolic extract indicated existence of polyphenols, such as flavonoids tannins, these constituents could be responsible for Carissa carandas's anxiolytic capacity. Preliminary photochemical present in petroleum ether, ethyl acetate, ethanol extracts of Carissa carandas leaves were screened using normal methods. Extracts' anticonvulsant potency was calculated using pentylenetetrazole (PTZ) in laboratory animal models with diazepam as control agent; sedative effect was tested using pentobarbitone, which causes sleep in mice. Samples were compared to standard medication Diazepam for effectiveness. Tentative phytochemical tests indicated existence of alkaloids, glycosides, tannins, and terpins. At dosage of 400 mg/kg, crude extracts of ethanolic, ethyl acetate, petroleum ether were found to substantially reduce extensor stupor, as well as provide protection against PTZ-induced convulsions. At dosage of 100 mg/kg in flexion, petroleum ether, ethyl acetate, ethanolic extract were found to be non-significant. Crude extracts of ethanolic, ethyl acetate, petroleum ether of Carissa carandas leaves were found to extend duration of sodium pentobarbital-induced hypnosis substantially (p 0.05).

Keywords: Carissa carandas, anticonvulsant, antianxiety, diazepam.