Abstract

A NOVEL, SENSITIVE AND VALIDATED UVSPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF RIBAVIRIN IN PURE AND PHARMACEUTICAL DOSAGE FORM

*R. Deepthi, G. Manasa, K.V.S. Bharat Kumar, K. Varuni and Y. Sravani

.

Abstract

Ribavirin is a nucleoside analogue used as an antiviral, anti-metabolite, anti-infective drug mostly used to treat Chronic Hepatitis-C.A specific and economic UV spectrophotometric method has been developed using Distilled Water as solvent at a predetermined λmax of 224nm. Beer limit was found in the range of 5-40 μg/mL and exhibited good correlation coefficient (R2 = 0.999) and the regression equation was found to be y=0.021x+0.077.This method was successfully applied in the determination of Ribavirin content in a marketed formulation from the local market and the results were in good agreement with the label claim.The method was validated according to the ICH guidelines for Range, linearity, Accuracy, Precision, Robustness, Ruggedness, LOD, LOQ and Sensitivity. The obtained results proved that the method can be employed for the routine analysis of Ribavirin in bulk as well as for formulation.

Keywords: Ribavirin, Method development, Validation, UV spectrophotometer, Precision, Accuracy.