Abstract

VACCINE DESIGN FOR HD AG-L AND HYPOTHETICAL PROTEIN OF HEPATITIS DELTA VIRUS

Mohammed Yousif Mohammed and Mona Mohamed Khaier*

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Abstract

Background: Hepatitis Delta Virus is responsible for many complications occurring during infection of Hepatitis B virus and responsible for serious damage to liver, most studies focus only on hepatitis B virus ignoring delta virus but its co-infection to liver make it very important in studies and research. Aim: in this study an epitopes vaccine was designed for Hepatitis Delta Virus (HDV) of the two protein present HD Ag and hypothetical protein using bioinformatics prediction tools. Methods: After retrieval of the two protein sequences from National Center for Biotechnology Information (NCBI) BCEpred and ABCpred for B cell epitopes and NetMHC 4 server for T-cell MHC class I and MHC class II was used. ProPred sever was used also to identify the antibody prediction epitopes for linear and discontinuous epitopes using IEDB Ellipro tool. Allergenicity was predicted by AllerTop server, antigenicity by Vaxijen 2 server and toxicity was predicted using Toxinpred. The 3D structure was modeled using I-Tasser server to visualize the two proteins. Docking process was achieved by ClusPro server to see how HDAg protein binds with the receptor of immune system. Results: BCEpred predicted 5 epitopes and ABCpred predicted 21 epitopes for B cell of HD Ag protein for hypothetical predicted 10 BCEpred epitopes and 11 ABCpred epitopes for hypothetical protein for T cell 16 epitopes were predicted by NeTMHC 4 MHC class I and 12 epitopes for MHC class II for ProPred of HDAg protein and 15 epitopes for MHC class I and 9 epitopes for MHC class II for hypothetical protein. All epitopes are antigenic and no allergy was detected plus not toxic effect was predicted. Concluding that these predicted epitopes can be used as a multi epitopes vaccine.

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