Abstract

EVALUATION OF SPONDIANTHUS PREUSII EFFECT ON MOVEMENT DIFFICULTY IN ANIMAL MODEL

Divine Oghenefejiro Akpovwovwo, Iyele Kamenebali, Ozakieoniso James Kemelayefa*

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Abstract

Background: Movement disorders populates among the advanced aged groups with some genetically enroot disease conditions such as the -cerebral autosomal dominant arteriopathy as well as -subcortical infarct and –leukoencephalopathy. No cure but management exist for theses neurological disorders by a way of symptomatic treatments, physiotherapeutic measures and brain stimulations. Medicinal plant has been prioritized on the bases of supplementary medicine, Spondianthus preusii represents one of the alternatives. Methods: Ethical approval was secured for animal research. The animal grouping includes: -A=Normal control; -B= standard control (bromocriptine 2.5mg/kg); -C= 100mg/kg, 200mg/kg and 400mg/kg Methanol fraction; -D= 100mg/kg, 200mg/kg, and 400mg/kg n-hexane fraction; -E= 100mg/kg, 200mg/kg and 400mg/kg of n-butanol fraction; -F= 100mg/kg, 200mg/kg and 400mg/kg ethylacetate fraction; -G= 100mg/kg, 200mg/kg and 400mg/kg aqueous fraction of S. presuii extract to animals respectively. The study was designed into groups as acute treated with aqueous, methanol, n-hexane, n-butanol, ethyl acetate fractions of S. preusii and sub-acute treated with n-butanol and n-hexane S. preusii. Grip strength determination: The grip strength test was manually formulated using 4.0mm wire of 20 cm length and was fixed to both ends of a wooden pole in the laboratory as part of the improvisation. The treated animals in respective groups were individually clamped into the set-up with their fore-arms and immediately timed for when it will let off the grip and such time values were recorded as grip strength within time range of 300 seconds After 30 minutes administration of chlorpromazine. Cataleptic evaluation: The various groups were treated respectively as started above. After 30 minutes administer chlorpromazine to all groups. Observe the animals for catalepsy 10, 30 and 60 minutes. Score the animals according to the criteria below: 0 = if animal moves normally when placed on the table; 1 = if animal moves only when touched; 2 = if animal fails to correct posture when fore paws are placed on the 3 cm high wooden block; 3 = if animal fails to remove fore paws when placed on the 9 cm high wooden block; 4= no movement at all. Result: The n-butanol, and n-hexane fractions of S.preusii suggested inhibitory potentials against the cataleptic inducing agent, chlorpromazine at mid and lower doses in both factions in acute and sub-acute studies. Conclusion: The plant fractions (n-butanol and n-hexane) has shown to potentiate anti-cataleptic effect.

Keywords: Catalepsy, spondianthus preusii, movement disorders.