Abstract

COMPARATIVE EVALUATION OF UV/VISIBLE AND HPLC ANALYTICAL METHODS WHEN APPLIED FOR ESTIMATION OF PENTOPRAZOLE SODIUM

Dr. Gayatri Barabde, *Aarti Babu Kokate and Tushar Gaikar

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Abstract

Simple and accurate spectrophotometric and HPLC method was developed for determination of Pantoprazole in lyophilized injection dosage form. The spectrophotometric method was developed by dissolving tablets in distilled water to make solution of 20ppm giving absorbance at 290nm. The experimental conditions were optimized and Beers law was obeyed over the applicable concentration range. The application of HPLC procedure depends on using a Thermo BDS Hypersil, C18 Column along with mobile phase consisting of Buffer pH 4 and acetonitrile (40: 60 v/v). Both techniques were applied successfully for analysis of Pantoprazole in different commercially available tablets and capsule. From the results obtained for both procedures percentage purity was found out.

Keywords: Pantoprazole Na, Spectrophotometric, HPLC, Method development, Validation.