Abstract

ACUTE ORAL TOXICITY OF PRAMEHAKULANTAKA RASA IN WISTER ALBINO RATS

Deepika Gupta*, Ram Kishor Joshi and Gaurav Bilwal

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Abstract

Background: A herbo-metallic Ayurvedic formulation Pramehakulantaka Rasa mentioned for the treatment of Prameha/Madhumeha in Ayurvedic text was subjected to Acute Oral Toxicity Study according to OECD Guideline 423 for evaluation of its safety. Material and Method: Two dose levels of 300 mg/kg and 2000 mg/kg of Pramehakulantaka Rasa were selected to evaluate its safety and hence were administered in two groups of three Wistar albino rats each. Each rat was under observation for 14 days. Observation: Each rat was observed for behavioral changes at 30min, 4hr, 24 hr, 48 hr, 1st week, and 2nd week in terms of changes in skin & fur, mucous membranes, for the appearance of any abnormal symptoms like salivation, lethargy, sleep, coma, concentration, tremor, diarrhea, morbidity, and motility. At the end of 14th day, hematological analysis was conducted. Results: Throughout 14 days of observation, no abnormal behavioral changes were noted in any of the rats also the values of hematological analysis remained under normal limits at the end of the 14th day. Conclusion: through the results obtained from the study, it could be concluded that the Pramehakulantaka Rasa has no toxic effect in Wister albino rats and hence can be used for further evaluation of its effects.

Keywords: Prameha, Madhumeha, Herbometallic formulation, Ayurvedic drug, Preclinical Study, Toxicity study, OECD Guideline 423.