Abstract

STUDY OF IN-VITRO ANTICOAGULANT ACTIVITY ON KAEMPFERIA ROTUNDA

Safeera Mayyeri*, Anjaly V., Asheeba Mohammed Anzari, Mohammed Ajmal O., Shahma Jabeen M., Lubna Nasrin C. P., Dr. Celestin Baboo R. V. and Dr. Sirajudheen M. K.

.

Abstract

Hemostasis is a process resulting from the interaction between coagulation and anticoagulants that keeps blood within the injured vascular system during an injury. Three main steps make up the delicate mechanism of hemostasis involves vasoconstriction, platelet plug temporarily blockage and blood clotting or fibrin clot formation. Anticoagulant medications are required for the long-term prevention of recurrences as well as the short-term therapy of arterial and venous thrombotic diseases. Folklore practitioners have claimed that Kaempferia rotunda has anticoagulant properties. The purpose of this study is to highlight the potential of Kaempferia rotunda, a member of Zingiberaceae family, as an anticoagulant agent. A suitable solvent, such as methanol, was used to prepare the rhizome extract using the Soxhlet extraction method, and platelet-poor plasma samples were used to measure the extract's anticoagulant activity using the APTT and PT assays. The outcomes were contrasted with those attained with conventional heparin. The maximum anticoagulant activity was shown by the platelet-poor plasma sample treated with the concentration 100 μg/ml (83.6 sec: APTT; 18.68 sec: PT).

Keywords: Kaempferia rotunda, Anticoagulant, Platelet-poor plasma, APTT, PT, Assays.