Abstract

UV-VISIBLE SPECTROSCOPY: A COMPREHENSIVE REVIEW ON INSTRUMENTATION

Chakravarthi K. S. S.*, K. B. Ilango, Antony Maria Thomas S., Kowsalya Devi M., Sudharshan N. and Swetha V.

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Abstract

The first spectroscopic scientist was Johannes Marcus Marci. Spectroscopy is a branch of science that commence with Isaac Newton splitting light with a prism and was called optics. It involves the determination of Electromagnetic Radiation which is absorbed or emitted when molecules or atoms or ions of the sample move from one energy state to another energy state. Principle involved in Ultraviolet- Visible spectroscopy is electronic transition which describes the excitation of an electron from ground state to the excited state. For the measurements in spectrophotometers, we need to use a narrow band of wavelength of light. For these filters and monochromator are used. A filter is a device which transmit radiation of some wavelength, but absorbs wholly or partially other wavelengths. The monochromator is used to convert the polychromatic light into desirable monochromatic light. Converting the light source into electrical signal is the main function of the detector. Detectors are instrument that are used for measurement and to convert transmitted or reflected light from the sample to signal.

Keywords: UV- Visible Spectroscopy; Electronic transition, Beer’s, and Lambert’s Law; Instrumentation; Photomultiplier tube.