Abstract

DEVELOPMENT OF COLORIMETRIC METHOD FOR THE ANALYSIS OF AMINOCAPROIC ACID USING DCQ

Shaza W. Shantier*, Elrasheed A. Gadkariem and Rashida Mohamed

Abstract

A simple, sensitive, accurate and cost-effective colorimetric method was developed for the analysis of aminocaproic acid in bulk and injectable forms. The developed method was based on the generation of a colored complex through utilizing the known reaction of 2, 6- dichloroquinone 4-chloroimide (DCQ) with phenols, primary and secondary amines. A colored product with λmax at 680nm was formed after heating aminocaproic acid with DCQ in dimethylsulfoxide (DMSO) for 10 minutes. Stoichiometry of the reaction was studied and revealed 1:1 ratio. Beer’s law was found to be valid over the concentration range 3-15μg/ml. Regression analysis showed good correlation coefficient (not less than 0.998) with detection limit and quantification limit of 1.51μg/ml and 5.00μg/ml respectively. The mean percentage recovery was found to be 100.30 ±1.84, n=4.

Keywords: Colorimetry; Aminocaproic acid; DCQ.