Abstract

ANTI-PROLIFERATIVE EFFECT OF EUCALYPTUS CAMALDULENSIS AGAINST EHRLICH ASCITES CARCINOMA (EAC) CELLS IN SWISS ALBINO MICE IN VIVO

Nabila Zein*, Enaiat K. Mohamed, Fatma ELZahraa ElSayed

Abstract

Eucalyptus camaldulensis (Myrtaceae) is a tall evergreen tree found commonly in Egypt. The Aim of our study To explore the in vivo antitumor effect of Eucalyptus camaldulensis stem bark methanol extract (ECME) against Ehrlich's ascites carcinoma (EAC) in Swiss albino mice. The antitumor activity of ECME was studied by estimating hematological parameters of experimental mice, the effects of ECME on the count of EAC cells and reduction in tumor volume were studied, Cytotoxicity evaluation of ECME using EAC cells was determined by MTT assay, Isolated DNA from Ehrlich ascites carcinoma cells in treated and untreated animals was used for DNA fragmentation assay on agarose gel (1.5%) electrophoresis. Treatment with ECME decreased EAC cell count and tumor volume for preventive and therapeutic groups. It restored the altered hematological parameters normal level In MTT assay there is significant decrease in absorbance of EAC cells between preventive and therapeutic groups compared to positive control group. DNA fragmentation assay of Ehrlich ascites carcinoma cells from treated animals illustrated apoptotic effect of the extract due to the ladder forming pattern this confirmed that ECME caused EAC cell death. The present work indicates that ECME possess significant antitumor and antioxidant activity in vivo.

Keywords: Eucalyptus camaldulensis, ECME, DNA fragmentation, EAC, MTT.