Abstract

ULTRA STRUCTURAL AND HISTOLOGICAL STUDIES ON MICE BONE UNDER THE INFLUENCE OF NICKEL SALT TOXICITY

Khalid H. Gathwan and Ibrahim Isam Al-Karkhi*

Abstract

Bone is essentially a heterogeneous system consisting of water, mineral and collagen which determine the physical properties of bone composite material. The decrease in bone weight was dose dependent after treatment with all the two salts of metal i.e. NiNO3 and NiSO4. Bone provides essential framework and rigidity to the body. The toxic substances affect the development of bone by impairing the availability of essential nutrients resulting in abnormal bone development and toxicity. The impact of nickel toxicity on bone of mice has been studied histochemically (under light and electron microscope) by using two nickel salts. Histopathologically, the necrosis to layers of decalcified bone i.e. periosteum, matrix and endosteum was observed with all two salts. The bone forming cells, lamellae and Haversian canals were also affected. The cortical width of bone section decreased as the dose of salts increased. These changes were also observed on samples of powdered dried bone of all groups with SEM. These changes were dose dependent and of the same order as mentioned above. The toxicity of nickel salts was in the order of NiSO4 > NiNO3.

Keywords: mice, nickel salts, SEM.