Abstract

EFFECT OF ZINC TREATMENT ON EXCESS FLUORIDE TREATED MICE WITH A SPECIAL REFERENCE TO SPERMATOGENIC METAPHASE

Kshinansu Samanta and Chandan Roy*

Abstract

Fluoride (F) toxicity results in reproductive failure in male. The present study was undertaken to see the ameliorative effect of Zn supplementation at the level of 2000 ppm in Fluoride (F) induced toxicity in male wister strain mice. The study was carried out by taking 10 animals for each group. One group treated with Fluoride (F) as well as another one treated as Zinc (Zn) supplemented Fluoride (F) treated experimental group and another group served as control. The experiment was carried out for 30 consecutive days. All mice were fed with crushed food pellets ad libitum. Fluoride (F) toxicity arrest the spermatogenesis at the level of metaphase as the metaphase index in fluoride (F) treated group is reduced by 45.86%. Zn supplementation at the level of 2000 ppm failed to increase the Zn content of testis and epididymis significantly and did not change the metaphase index in Fluoride treated mice. However, supplementation of Zn (2000 ppm) can reduce the Fluoride content of bone (femur) (P < 0.001) in Fluoride treated group. It has been suggested that excess Zn at the level of 2000 ppm reduced the fluoride load of bone however unable to prevent the fluoride induced reproductive toxicity in male.

Keywords: Fluoride, Toxicity, Zinc supplementation, Metaphase Index, Femur.