Abstract

COMPARATIVE PHYTOCHEMICAL STUDY OF HEARTWOOD VERSUS SMALL BRANCHES OF DALBERGIA SISSOO LINN USING HPTLC-UV DETECTION METHOD

S. C. Verma*, S. Subhani, E. Vashishth, R. Singh, P. Pant, M. M. Padhi and A. Kumar

Abstract

Dalbergia sissoo Linn is Indian rosewood which is a deciduous forest tree. It is natively found in Indian subcontinent. It is called as Shisham which is best known premier timber tree. It is also used as fuel wood with its multiple product uses and agro-forestry application, it is consider as best timber wood tree. D. sissoo is a widely growing plant which is used traditionally as anti-inflammatory, antipyretic, analgesic, anti-oxidant, anti-diabetic and antimicrobial agent. Several phytoconstituents have been isolated and identified from different parts of the plant belonging to the category of alkaloids, glycosides, flavanols, tannins, saponins, sterols and terpenoids. Our results revealed that the chromatographic fingerprint combined with similarity measurement could efficiently identify and distinguish D. sissoo heartwood and small branches. The phytochemical fingerprint profiling of heartwood and small branches of D. sissoo were found similar as an official part of D. sissoo plant i.e. heartwood, therefore small branches may be used in place of heartwood and vice-versa after comparison and confirmation of same pharmacological activities. The method can also be used for identification of different D. sissoo species and adulterants.

Keywords: Dalbergia sissoo., HPTLC–UV detection, phytochemical fingerprint profiling analysis.