Abstract

COMPARISON STUDY OF THE ANTIMICROBIAL ACTIVITY OF SEED PROTEIN EXTRACTS FROM FOUR MEDICINAL PLANTS AGAINST XANTHOMONAS OXANOPODIS VER. PUNICAE.

Shantaveera Swamy H. M*, Vijay Kumara Swamy H. V and Preeti Upadhya

Abstract

The present study was conducted to evaluate the efficacy of antimicrobial peptides from four medicinal plants, i.e, Glycine max, Crotalaria juncea, Adenanthera pavonina and Lawsonia inermis against Xanthomonas oxanopodis pv. punicae which is an obligatory parasite of Pomegranate - Punica granatum. The extraction was carried out in Potassium phosphate buffer, Sodium phosphate buffer and Sodium acetate buffer. The antimicrobial activities of these plants were determined by microbiological technique using disc diffusion method against Xanthomonas. All the extracts exhibited antibacterial activity against Xanthomonas and the stronger antibacterial activity was observed by Crotalaria juncea extract against Xanthomonas with 31±1.5mm zone of inhibition. Our results indicated that the Crotalaria juncea is a rich source for antimicrobial peptides. The detach of Vance length of antimicrobial activity of the crude extraction from medicinal plants and presented in this paper.

Keywords: Antimicrobial activity, medicinal plants, Xanthomonas oxanopodis pv. punicae.